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These LsBoost algorithm-based models exhibited excellent precision (0.82 ~ 1.00) and demonstrated robust predictive potential as evidenced by the similar MSE, MAE, RMSE, and R 2 values between training and validation sets (Table S4)

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Roughly 12,000 beads were combined with the above ligation mix and incubated for 1 hour at 37C in an Eppendorf ThermoMixer (15 second alternative mixing at 1800 rpm)

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