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doi: 10.1016/j.jaad.2014.03.019 140 MashalyMEKotbHMAbdinAAHedyaSE

The solution was allowed to react for 30 min at 60C followed by injecting into the HPLC at a flow rate of 0.8 mL/min, the separation was performed at ambient temperature, and the detection was carried at 280 nm