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If I wanted to investigate the interaction of two levels (high and low) of two different factors (protein dose and carcinogen dose) I would have used a 22 design with four groups: Low-aflatoxin, low-protein Low-aflatoxin, high-protein High-aflatoxin, low-protein High-aflatoxin, high protein I would be able to depict the results in a table looking something like this: Yet Campbell only describes using the second and third groups