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The inactivation or silencing of Gpx4 gene expression can result in the death of the embryo, possibly due to developmental defects in the brain ( GPX4 are associated with sedaghatnia-type spondylometaphyseal chondrodysplasia, which causes bone, heart, and brain abnormalities and increased cell death (Smith et al., 2014)

eLife 8:9278

Next, cells were cultured during 2 days in induction medium containing medium 1 plus 0.5 M dexamethasone, 0.5 mM IBMX, 0.125 M indomethacin and 1 M rosiglitazone